DEDUCT | Database of Endocrine Disrupting chemicals and their Toxicity profiles

Methotrexate

Curated chemical-phenotype interactions from CTD

GO ID	GO name	Interaction type	Reference
GO:0000084	Mitotic s phase	Increases phenotype	PMID:31170432
GO:0001541	Ovarian follicle development	Decreases phenotype	PMID:33745333
GO:0001552	Ovarian follicle atresia	Increases phenotype	PMID:37866152
GO:0001894	Tissue homeostasis	Decreases phenotype	PMID:27605418
GO:0002523	Leukocyte migration involved in inflammatory response	Increases phenotype	PMID:33530773; PMID:33530773; PMID:36890697; PMID:33530773; PMID:37312617; PMID:33745333; PMID:33745333; PMID:37866152; PMID:34802285; PMID:37866152
GO:0002693	Positive regulation of cellular extravasation	Increases phenotype	PMID:30025850
GO:0003331	Positive regulation of extracellular matrix constituent secretion	Increases phenotype	PMID:27605418
GO:0004021	L-alanine:2-oxoglutarate aminotransferase activity	Increases phenotype	PMID:39988050
GO:0004035	Alkaline phosphatase activity	Increases phenotype	PMID:28414158
GO:0004069	L-aspartate:2-oxoglutarate aminotransferase activity	Increases phenotype	PMID:28414158; PMID:28414158; PMID:28479099; PMID:33530773; PMID:39988050; PMID:28479099; PMID:33530773; PMID:33845614; PMID:33845614; PMID:34802285; PMID:34802285; PMID:39988050
GO:0004364	Glutathione transferase activity	Decreases phenotype	PMID:34737150; PMID:37341015
GO:0004457	Lactate dehydrogenase activity	Increases phenotype	PMID:28414158; PMID:28414158; PMID:28479099; PMID:28479099
GO:0004602	Glutathione peroxidase activity	Decreases phenotype	PMID:28479099; PMID:28479099; PMID:33530773; PMID:31975554; PMID:33530773; PMID:33845614; PMID:33845614; PMID:34802285; PMID:34802285; PMID:37866152
GO:0004784	Superoxide dismutase activity	Affects phenotype	PMID:28414158; PMID:28414158; PMID:28479099; PMID:31362085; PMID:33530773; PMID:39988050; PMID:28479099; PMID:31362085; PMID:31975554; PMID:31975554; PMID:39043351; PMID:33530773; PMID:34737150; PMID:34802285; PMID:37341015; PMID:37866152; PMID:37939893; PMID:39043351; PMID:39988050
GO:0005980	Glycogen catabolic process	Increases phenotype	PMID:7713362
GO:0006020	Inositol metabolic process	Affects phenotype	PMID:36531023
GO:0006163	Purine nucleotide metabolic process	Affects phenotype	PMID:36531023
GO:0006220	Pyrimidine nucleotide metabolic process	Affects phenotype	PMID:36531023
GO:0006304	Dna modification	Increases phenotype	PMID:37450014
GO:0006382	Adenosine to inosine editing	Increases phenotype	PMID:28188287
GO:0006520	Amino acid metabolic process	Affects phenotype	PMID:36531023
GO:0006556	S-adenosylmethionine biosynthetic process	Decreases phenotype	PMID:22193356
GO:0006580	Ethanolamine metabolic process	Affects phenotype	PMID:36531023
GO:0006629	Lipid metabolic process	Affects phenotype	PMID:35156134
GO:0006749	Glutathione metabolic process	Increases phenotype	PMID:25009784; PMID:26517535; PMID:29069929; PMID:29069929; PMID:33720507; PMID:29069929; PMID:36249937; PMID:32061742; PMID:33720507; PMID:33745333; PMID:33745333; PMID:37866152; PMID:34737150; PMID:34802285; PMID:36249937; PMID:37866152
GO:0006750	Glutathione biosynthetic process	Decreases phenotype	PMID:28414158; PMID:28414158; PMID:28479099; PMID:31362085; PMID:28479099; PMID:30426164; PMID:31362085
GO:0006761	Dihydrofolate biosynthetic process	Decreases phenotype	PMID:22484375
GO:0006809	Nitric oxide biosynthetic process	Increases phenotype	PMID:28414158
GO:0006855	Xenobiotic transmembrane transport	Decreases phenotype	PMID:15897250; PMID:35019195
GO:0006874	Cellular calcium ion homeostasis	Affects phenotype	PMID:35156134
GO:0006915	Apoptotic process	Increases phenotype	PMID:10921050; PMID:23836430; PMID:25575725; PMID:37312617; PMID:37341015; PMID:37450014; PMID:8554984
GO:0006919	Activation of cysteine-type endopeptidase activity involved in apoptotic process	Increases phenotype	PMID:34350509
GO:0006954	Inflammatory response	Increases phenotype	PMID:27613480; PMID:29216766; PMID:34737150; PMID:37341015
GO:0006974	Cellular response to dna damage stimulus	Increases phenotype	PMID:29069929
GO:0007033	Vacuole organization	Increases phenotype	PMID:33530773
GO:0007283	Spermatogenesis	Decreases phenotype	PMID:31975554; PMID:32666843; PMID:39043351
GO:0007417	Central nervous system development	Affects phenotype	PMID:23836430
GO:0007616	Long-term memory	Decreases phenotype	PMID:31651976
GO:0008202	Steroid metabolic process	Affects phenotype	PMID:36531023
GO:0008210	Estrogen metabolic process	Affects phenotype	PMID:37866152
GO:0008219	Cell death	Increases phenotype	PMID:20072153; PMID:24518598; PMID:25373508; PMID:27099147; PMID:27377006; PMID:34737150
GO:0008283	Cell population proliferation	Decreases phenotype	PMID:19435820; PMID:23836430; PMID:24930440; PMID:27377006
GO:0008284	Positive regulation of cell population proliferation	Decreases phenotype	PMID:15897250; PMID:18623116; PMID:22580740; PMID:24657277
GO:0008285	Negative regulation of cell population proliferation	Increases phenotype	PMID:30502384; PMID:31563593; PMID:32259555; PMID:32505695; PMID:35779630
GO:0008610	Lipid biosynthetic process	Affects phenotype	PMID:30426164; PMID:32535746
GO:0008654	Phospholipid biosynthetic process	Affects phenotype	PMID:30426164
GO:0009112	Nucleobase metabolic process	Affects phenotype	PMID:25145655
GO:0009116	Nucleoside metabolic process	Affects phenotype	PMID:25145655
GO:0009117	Nucleotide metabolic process	Affects phenotype	PMID:34953855
GO:0009119	Ribonucleoside metabolic process	Affects phenotype	PMID:36531023
GO:0009211	Pyrimidine deoxyribonucleoside triphosphate metabolic process	Affects phenotype	PMID:22484375
GO:0009219	Pyrimidine deoxyribonucleotide metabolic process	Affects phenotype	PMID:19593106
GO:0009308	Amine metabolic process	Affects phenotype	PMID:36531023
GO:0009410	Response to xenobiotic stimulus	Decreases phenotype	PMID:23675469
GO:0010718	Positive regulation of epithelial to mesenchymal transition	Increases phenotype	PMID:31170432
GO:0010744	Positive regulation of macrophage derived foam cell differentiation	Affects phenotype	PMID:19035488
GO:0010942	Positive regulation of cell death	Increases phenotype	PMID:16609701; PMID:17003774; PMID:17379860; PMID:19192274; PMID:18694510; PMID:18694510; PMID:20515499; PMID:19548002; PMID:20515499; PMID:20668838; PMID:22954684; PMID:20705681; PMID:21625222; PMID:22524704; PMID:23675469; PMID:23675469; PMID:23911960; PMID:23725749; PMID:23792648; PMID:23799623; PMID:23911960; PMID:24657277; PMID:24688052; PMID:24736981; PMID:25078587; PMID:27605418; PMID:30025850
GO:0015723	Bilirubin transport	Affects phenotype	PMID:33530773
GO:0015884	Folic acid transport	Affects phenotype	PMID:18207340
GO:0016042	Lipid catabolic process	Increases phenotype	PMID:27377006; PMID:28414158; PMID:28414158; PMID:28479099; PMID:31362085; PMID:28479099; PMID:29069929; PMID:29069929; PMID:29216766; PMID:29069929; PMID:33720507; PMID:29216766; PMID:31362085; PMID:31975554; PMID:33720507; PMID:34737150
GO:0016049	Cell growth	Decreases phenotype	PMID:27166432; PMID:30426164
GO:0018158	Protein oxidation	Increases phenotype	PMID:25009784; PMID:29069929
GO:0019395	Fatty acid oxidation	Increases phenotype	PMID:32535746; PMID:38676573
GO:0019627	Urea metabolic process	Affects phenotype	PMID:33530773; PMID:33530773; PMID:37312617; PMID:37312617
GO:0019646	Aerobic electron transport chain	Affects phenotype	PMID:38676573
GO:0019674	Nad metabolic process	Affects phenotype	PMID:36531023
GO:0019752	Carboxylic acid metabolic process	Affects phenotype	PMID:36531023
GO:0019919	Peptidyl-arginine methylation, to asymmetrical-dimethyl arginine	Increases phenotype	PMID:36890697
GO:0030263	Apoptotic chromosome condensation	Increases phenotype	PMID:32666843; PMID:39988050
GO:0034440	Lipid oxidation	Increases phenotype	PMID:25009784; PMID:26517535; PMID:32061742; PMID:33745333; PMID:34802285; PMID:36249937; PMID:37341015; PMID:37450014; PMID:37450014; PMID:39043351; PMID:39043351
GO:0035809	Regulation of urine volume	Decreases phenotype	PMID:31039415
GO:0035812	Renal sodium excretion	Affects phenotype	PMID:31039415
GO:0036211	Protein modification process	Affects phenotype	PMID:27038877
GO:0040015	Negative regulation of multicellular organism growth	Increases phenotype	PMID:32061742
GO:0042307	Positive regulation of protein import into nucleus	Increases phenotype	PMID:29935243
GO:0042428	Serotonin metabolic process	Affects phenotype	PMID:37927177
GO:0042448	Progesterone metabolic process	Affects phenotype	PMID:37866152
GO:0042554	Superoxide anion generation	Affects phenotype	PMID:30426164
GO:0043065	Positive regulation of apoptotic process	Increases phenotype	PMID:16520895; PMID:18483502; PMID:18803016; PMID:18803016; PMID:22484375; PMID:19593106; PMID:21114963; PMID:21114963; PMID:23799623; PMID:21819747; PMID:22010212; PMID:22484375; PMID:22484375; PMID:23675469; PMID:23675469; PMID:23799623; PMID:23838799; PMID:24444433; PMID:24935781; PMID:24935781; PMID:24967384; PMID:24967384; PMID:25330300; PMID:30502384
GO:0043517	Positive regulation of dna damage response, signal transduction by p53 class mediator	Decreases phenotype	PMID:16598767
GO:0044237	Cellular metabolic process	Decreases phenotype	PMID:31362085; PMID:32535746; PMID:34762139; PMID:34953855; PMID:35156134; PMID:36531023
GO:0044242	Cellular lipid catabolic process	Increases phenotype	PMID:23424212
GO:0044338	Canonical wnt signaling pathway involved in mesenchymal stem cell differentiation	Increases phenotype	PMID:25187349
GO:0045429	Positive regulation of nitric oxide biosynthetic process	Increases phenotype	PMID:32061742
GO:0045454	Cell redox homeostasis	Increases phenotype	PMID:27450487
GO:0045930	Negative regulation of mitotic cell cycle	Increases phenotype	PMID:19548002; PMID:22484375; PMID:23799623; PMID:24935781
GO:0045931	Positive regulation of mitotic cell cycle	Decreases phenotype	PMID:18483502
GO:0046034	Atp metabolic process	Affects phenotype	PMID:32535746; PMID:34953855
GO:0046169	Methanol biosynthetic process	Increases phenotype	PMID:25009784
GO:0046209	Nitric oxide metabolic process	Affects phenotype	PMID:33720507; PMID:36249937
GO:0046330	Positive regulation of jnk cascade	Increases phenotype	PMID:18483502; PMID:22183962
GO:0046449	Creatinine metabolic process	Affects phenotype	PMID:29216766; PMID:29216766; PMID:33530773; PMID:36249937; PMID:37312617; PMID:33530773; PMID:33845614; PMID:36249937; PMID:37312617
GO:0046460	Neutral lipid biosynthetic process	Increases phenotype	PMID:32535746
GO:0046470	Phosphatidylcholine metabolic process	Affects phenotype	PMID:36531023
GO:0046655	Folic acid metabolic process	Decreases phenotype	PMID:19152916; PMID:22193356; PMID:24657277; PMID:25145655
GO:0048266	Behavioral response to pain	Increases phenotype	PMID:32061742
GO:0048873	Homeostasis of number of cells within a tissue	Decreases phenotype	PMID:35779630
GO:0051246	Regulation of protein metabolic process	Decreases phenotype	PMID:24712521
GO:0051276	Chromosome organization	Affects phenotype	PMID:22806879
GO:0051782	Negative regulation of cell division	Increases phenotype	PMID:35779630
GO:0051881	Regulation of mitochondrial membrane potential	Affects phenotype	PMID:30426164; PMID:35156134
GO:0051958	Methotrexate transport	Decreases phenotype	PMID:18499665
GO:0055074	Calcium ion homeostasis	Affects phenotype	PMID:30426164
GO:0060073	Micturition	Decreases phenotype	PMID:31039415
GO:0061003	Positive regulation of dendritic spine morphogenesis	Increases phenotype	PMID:31651976
GO:0061370	Testosterone biosynthetic process	Affects phenotype	PMID:31975554; PMID:32666843; PMID:32666843; PMID:34340576; PMID:37450014; PMID:34340576; PMID:37450014
GO:0070237	Positive regulation of activation-induced cell death of t cells	Increases phenotype	PMID:22183962
GO:0070265	Necrotic cell death	Increases phenotype	PMID:29216766; PMID:31362085
GO:0070318	Positive regulation of g0 to g1 transition	Decreases phenotype	PMID:24736981
GO:0070994	Detection of oxidative stress	Affects phenotype	PMID:32666843; PMID:33530773; PMID:33530773; PMID:36890697; PMID:39988050; PMID:33530773; PMID:37312617; PMID:33530773; PMID:39988050; PMID:33745333; PMID:33745333; PMID:37866152; PMID:33845614; PMID:33845614; PMID:34802285; PMID:34802285; PMID:36482745; PMID:36890697; PMID:37312617; PMID:37341015; PMID:37866152; PMID:37927177; PMID:39988050
GO:0071169	Establishment of protein localization to chromatin	Increases phenotype	PMID:35842499
GO:0071414	Cellular response to methotrexate	Increases phenotype	PMID:22806879
GO:0072593	Reactive oxygen species metabolic process	Affects phenotype	PMID:35156134
GO:0072677	Eosinophil migration	Increases phenotype	PMID:34737150
GO:0090023	Positive regulation of neutrophil chemotaxis	Increases phenotype	PMID:32061742
GO:0097327	Response to antineoplastic agent	Affects phenotype	PMID:20705681; PMID:21625222; PMID:24736981; PMID:25078587
GO:0098657	Import into cell	Affects phenotype	PMID:38101600
GO:0140042	Lipid droplet formation	Increases phenotype	PMID:34802285
GO:0150020	Basal dendrite arborization	Decreases phenotype	PMID:31651976
GO:0150023	Apical dendrite arborization	Decreases phenotype	PMID:31651976
GO:1901670	Negative regulation of superoxide dismutase activity	Increases phenotype	PMID:32061742
GO:1901671	Positive regulation of superoxide dismutase activity	Increases phenotype	PMID:25009784
GO:1901687	Glutathione derivative biosynthetic process	Increases phenotype	PMID:25009784
GO:1901727	Positive regulation of histone deacetylase activity	Increases phenotype	PMID:21114963
GO:1901991	Negative regulation of mitotic cell cycle phase transition	Increases phenotype	PMID:31170432
GO:1902512	Positive regulation of apoptotic dna fragmentation	Increases phenotype	PMID:16520895
GO:1903284	Positive regulation of glutathione peroxidase activity	Affects phenotype	PMID:25009784; PMID:26517535
GO:1903409	Reactive oxygen species biosynthetic process	Affects phenotype	PMID:30426164
GO:1903428	Positive regulation of reactive oxygen species biosynthetic process	Increases phenotype	PMID:22183962; PMID:24967384; PMID:25330300
GO:1904179	Positive regulation of adipose tissue development	Increases phenotype	PMID:25187349
GO:1905517	Macrophage migration	Increases phenotype	PMID:36482745
GO:2000774	Positive regulation of cellular senescence	Increases phenotype	PMID:21678067
GO:2001170	Negative regulation of atp biosynthetic process	Increases phenotype	PMID:33147516

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We have built a comprehensive resource which compiles potential endocrine disrupting chemicals (EDCs) based on the observed adverse effects or endocrine-mediated endpoints in published experiments on humans or rodents to support basic research. We are not responsible for any errors or omissions in the published research articles or supporting literature on potential EDCs compiled in this resource. Users are advised to exercise their own judgement on the weight of evidence for potential EDCs compiled in this resource. Importantly, our sole goal to build this resource on potential EDCs is to enable future basic research towards better understanding of the systems-level perturbations upon chemical exposure rather than influencing regulatory advice on chemical use.